NON-PROPAGATED CORTICAL REACTIONS INDUCED BY THE DIVALENT IONOPHORE A23187 IN EGGS OF THE SEA-URCHIN, LYTECHINUS-VARIEGATUS

被引:33
作者
CHAMBERS, EL [1 ]
HINKLEY, RE [1 ]
机构
[1] UNIV MIAMI,SCH MED,DEPT BIOPHYS,MIAMI,FL 33101
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
D O I
10.1016/0014-4827(79)90221-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The divalent ionophore A23187 can induce a non-propagated discharge of cortical granules in sea urchin eggs resulting in the elevation of partial fertilization membranes. This occurs when unfertilized eggs (1) partially overlap the margin of a solid film of the ionophore; (2) touch a Sephadex bead previously infiltrated with the ionophore; or (3) are exposed to solutions of ionophore for short periods (15-30 sec). These results demonstrate that the release of cortical granules does not automatically induce the discharge of neighboring granules. © 1979.
引用
收藏
页码:441 / 446
页数:6
相关论文
共 29 条
[1]  
ALLEN RD, 1954, EXP CELL RES, V6, P412
[2]   ROLE OF DIVALENT-CATIONS IN ACTIVATION OF SEA-URCHIN EGG .1. EFFECT FERTILIZATION ON DIVALENT-CATION CONTENT [J].
AZARNIA, R ;
CHAMBERS, EL .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1976, 198 (01) :65-77
[3]   PROCEDURE FOR EMBEDDING IN SITU SELECTED CELLS CULTURED IN VITRO [J].
BRINKLEY, BR ;
MURPHY, P ;
RICHARDSON, LC .
JOURNAL OF CELL BIOLOGY, 1967, 35 (01) :279-+
[4]  
CHAMBERS EE, UNPUBLISHED
[5]   MEMBRANE-POTENTIAL, ACTION POTENTIAL AND ACTIVATION POTENTIAL OF EGGS OF THE SEA-URCHIN, LYTECHINUS-VARIEGATUS [J].
CHAMBERS, EL ;
DEARMENDI, J .
EXPERIMENTAL CELL RESEARCH, 1979, 122 (01) :203-218
[6]   ACTIVATION OF SEA-URCHIN EGGS BY DIVALENT IONOPHORE-A23187 AND IONOPPHORE-X-537A [J].
CHAMBERS, EL ;
PRESSMAN, BC ;
ROSE, B .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 60 (01) :126-132
[7]  
CHAMBERS EL, 1978, J CELL BIOL, V79, pA162
[8]  
CHAMBERS EL, 1974, J CELL BIOL, V63, pA56
[9]  
CHAMBERS EL, 1974, BIOL BULL, V147, P471
[10]   ISOLATION AND CHARACTERIZATION OF PLASMA MEMBRANE-ASSOCIATED CORTICAL GRANULES FROM SEA-URCHIN EGGS [J].
DETERING, NK ;
DECKER, GL ;
SCHMELL, ED ;
LENNARZ, WJ .
JOURNAL OF CELL BIOLOGY, 1977, 75 (03) :899-914