MAP-BASED CLONING OF A GENE CONTROLLING OMEGA-3-FATTY-ACID DESATURATION IN ARABIDOPSIS

被引：401
作者：
ARONDEL, V
LEMIEUX, B
HWANG, I
GIBSON, S
GOODMAN, HM
SOMERVILLE, CR
机构：
[1] MICHIGAN STATE UNIV, US DOE, PLANT RES LAB, E LANSING, MI 48824 USA
[2] YORK UNIV, DEPT BIOL, N YORK M3J 1P3, ONTARIO, CANADA
[3] HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA
[4] MASSACHUSETTS GEN HOSP, DEPT MOLEC BIOL, BOSTON, MA 02114 USA
来源：
SCIENCE | 1992年 / 258卷 / 5086期
关键词：
D O I：
10.1126/science.1455229
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.
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页码：1353 / 1355
页数：3
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