2-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF ENDOPOLYGALACTURONASES PRODUCED BY KLUYVEROMYCES-MARXIANUS

The high resolution technique of two‐dimensional electrophoresis is used to establish the endopolygalacturonase isoenzyme profile of Kluyveromyces marxianus, strain NCYC 587. We show that the use of this technique in nondenaturing conditions represents a powerful procedure for resolving the isoenzyme population and detecting their endopolygalacturonase activities. The enzyme staining method described is at least as sensitive as silver staining. The purified enzyme was shown to consist of nine isoenzymes. Their pIs ranged from 5.6 to 6.5 and they appeared to have similar molecular weights in the region of 43,000 daltons. Copyright © 1990, Wiley Blackwell. All rights reserved