SOLID-PHASE RADIOIMMUNOASSAY FOR HEPATITIS BE ANTIGEN (HBEAG)

被引：2

作者：

BLUM, HE ^{[1
]}

DOLKEN, G ^{[1
]}

GEROK, W ^{[1
]}

机构：

[1] UNIV FREIBURG,MED KLIN,D-7800 FREIBURG,FED REP GER

来源：

KLINISCHE WOCHENSCHRIFT | 1979年 / 57卷 / 20期

关键词：

anti-HBe; HBeAg; HBsAg; Hepatitis B; Solid-Phase Radioimmunoassay;

D O I：

10.1007/BF01481494

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations. © 1979 Springer-Verlag.

引用

页码：1129 / 1132

页数：4

共 21 条

[1] TYPE B-HEPATITIS - INFECTIVITY OF BLOOD POSITIVE FOR E ANTIGEN AND DNA-POLYMERASE AFTER ACCIDENTAL NEEDLESTICK EXPOSURE [J].