FORMATION AND PROPERTIES OF THIN-WALLED PHOSPHOLIPID VESICLES

被引：541

作者：

REEVES, JP

DOWBEN, RM

机构：

[1] Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1969年 / 73卷 / 01期

关键词：

D O I：

10.1002/jcp.1040730108

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Large numbers of thin‐walled vesicles, 0.5 to 10 μ in diameter, can be formed by permitting a thinly spread layer of hydrated phospholipids to swell slowly in distilled water or an aqueous solution of nonelectrolytes. Electron micrographs and phospholipid analyses indicate that the walls consist of a single or a few bilayers. The vesicles can be centrifuged and resuspended in another medium, making them a useful system for studying permeability. The osmolarity of the solution in the interior of the vesicles can be estimated by immersion refractometry. The osmolarity of the internal aqueous phase is linearly related to the osmolarity of the external medium. Copyright © 1969 Wiley‐Liss, Inc.

引用

页码：49 / &

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