HIGHLY SENSITIVE AND SPECIFIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF 7-HYDROXYWARFARIN, A MARKER FOR HUMAN CYTOCHROME P-4502C9 ACTIVITY

被引：38

作者：

LANG, D ^{[1
]}

BOCKER, R ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG,DEPT PHARMACOL & TOXICOL,D-91054 ERLANGEN,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00222-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of 7-hydroxywarfarin in incubations of (S)-warfarin with human liver microsomes reflects their cytochrome P-4502C9 activity. This paper describes a rapid high-performance liquid chromatographic method for the determination of 7-hydroxywarfarin with high sensitivity, selectivity, and a simple sample clean-up procedure. Separation was achieved with a C-18 reversed-phase column and quantification by fluorometric defection. The method employs an internal standard resulting in good accuracy and precision. The limit of detection is 150 fmol for 7-hydroxywarfarin.

引用

页码：305 / 309

页数：5

共 16 条

[1] STEREOSPECIFIC FLUORESCENCE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF WARFARIN AND ITS METABOLITES IN PLASMA AND URINE [J].

BANFIELD, C ;

ROWLAND, M .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1984, 73 (10) :1392-1396

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

BUSCH ED, 1983, BIOMED MASS SPECTROM, V10, P295

[4] HIGH-YIELD SYNTHESIS OF WARFARIN AND ITS PHENOLIC METABOLITES - NEW COMPOUNDS [J].

BUSH, E ;

TRAGER, WF .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1983, 72 (07) :830-831

[5]

FASCO MJ, 1978, J BIOL CHEM, V253, P7813

[6] BIOCHEMICAL APPLICATIONS OF A QUANTITATIVE HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC ASSAY OF WARFARIN AND ITS METABOLITES [J].

FASCO, MJ ;

PIPER, LJ ;

KAMINSKY, LS .

JOURNAL OF CHROMATOGRAPHY, 1977, 131 (JAN21) :365-373

[7]

Guengerich FP, 1989, PRINCIPLES METHODS T, P777

[8]

HERMODSON MA, 1971, J MED CHEM, V14, P167

[9]

KAMINSKY LS, 1980, J BIOL CHEM, V255, P85

[10] CHEMICAL SYNTHESIS, ABSOLUTE-CONFIGURATION, AND STEREOCHEMISTRY OF FORMATION OF 10-HYDROXYWARFARIN - A MAJOR OXIDATIVE METABOLITE OF (+)-(R)-WARFARIN FROM HEPATIC-MICROSOMAL PREPARATIONS [J].

LAWRENCE, RF ;

RETTIE, AE ;

EDDY, AC ;

TRAGER, WF .

CHIRALITY, 1990, 2 (02) :96-105

← 1 2 →