Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary

被引:5
作者
Kim, Dae-Jung [1 ]
Park, Chae-Won [2 ]
Byambaragchaa, Munkhzaya [2 ]
Kim, Shin-Kwon [1 ]
Lee, Bae-Ik [1 ]
Hwang, Hyung-Kyu [1 ]
Myeong, Jeong-In [1 ]
Hong, Sun-Mee [3 ]
Kang, Myung-Hwa [4 ]
Min, Kwan-Sik [2 ]
机构
[1] NIFS, Aquaculture Res Div, Busan 46083, South Korea
[2] Hankyong Natl Engn, Inst Genet Engn, Grad Sch Future Convergence Technol, Anim Biotechnol, Anseong 17579, South Korea
[3] Inst Gyeongbuk Marine Bioind, Dept Res & Dev, Ulgin 36315, South Korea
[4] Hoseo Univ, Dept Food Sci & Nutr, Asan 31499, South Korea
来源
DATA IN BRIEF | 2016年 / 8卷
关键词
Japanese eel; FSH; Monoclonal Antibody;
D O I
10.1016/j.dib.2016.05.069
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [l], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary. (C) 2016 The Authors. Published by Elsevier lnc.
引用
收藏
页码:404 / 410
页数:7
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