DYNAMICS OF CELL-MEMBRANE PERMEABILITY CHANGES AT SUPRAPHYSIOLOGICAL TEMPERATURES

被引:115
作者
BISCHOF, JC
PADANILAM, J
HOLMES, WH
EZZELL, RM
LEE, RC
TOMPKINS, RG
YARMUSH, ML
TONER, M
机构
[1] MASSACHUSETTS GEN HOSP, SURG SERV, BOSTON, MA 02114 USA
[2] HARVARD UNIV, SCH MED, SHRINERS BURN INST, BOSTON, MA USA
[3] HARVARD UNIV, SCH MED, DEPT SURG, BOSTON, MA 02115 USA
[4] MIT, DEPT MECH ENGN, CAMBRIDGE, MA 02139 USA
[5] UNIV CHICAGO, DEPT SURG, CHICAGO, IL 60637 USA
[6] RUTGERS STATE UNIV, DEPT CHEM & BIOCHEM ENGN, PISCATAWAY, NJ USA
关键词
D O I
10.1016/S0006-3495(95)80445-5
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A quantitative fluorescent microscopy system was developed to characterize, in real time, the effects of supraphysiological temperatures between 37 degrees and 70 degrees C on the plasma membrane of mouse 3T3 fibroblasts and isolated rat skeletal muscle cells. Membrane permeability was assessed by monitoring the leakage as a function of time of the fluorescent membrane integrity probe calcein. The kinetics of dye leakage increased with increasing temperature in both the 3T3 fibroblasts and the skeletal muscle cells. Analytical solutions derived from a two-compartment transport model showed that, for both cell types, a time-dependent permeability assumption provided a statistically better fit of the model predictions to the data than a constant permeability assumption. This finding suggests that the plasma membrane integrity is continuously being compromised while cells are subjected to supraphysiological temperatures.
引用
收藏
页码:2608 / 2614
页数:7
相关论文
共 33 条
[1]  
Bevington P. R., 1992, DATA REDUCTION ERROR
[2]   A DIRECT CORRELATION BETWEEN HYPERTHERMIA-INDUCED MEMBRANE BLEBBING AND SURVIVAL IN SYNCHRONOUS G1 CHO CELLS [J].
BORRELLI, MJ ;
WONG, RSL ;
DEWEY, WC .
JOURNAL OF CELLULAR PHYSIOLOGY, 1986, 126 (02) :181-190
[3]  
COSMAN MD, 1989, CRYO-LETT, V10, P17
[4]  
COSS RA, 1982, CANCER RES, V42, P1059
[5]  
CRAVALHO EG, 1992, ELECT TRAUMA PATHOPH, P281
[6]   THE SEARCH FOR CRITICAL CELLULAR TARGETS DAMAGED BY HEAT [J].
DEWEY, WC .
RADIATION RESEARCH, 1989, 120 (02) :191-204
[7]   THERMAL-INSTABILITY OF RED BLOOD-CELL MEMBRANE BILAYERS - TEMPERATURE-DEPENDENCE OF HEMOLYSIS [J].
GERSHFELD, NL ;
MURAYAMA, M .
JOURNAL OF MEMBRANE BIOLOGY, 1988, 101 (01) :67-72
[8]  
HANSSON LC, 1989, BIOCHIM BIOPHYS ACTA, V979, P166
[9]  
HENRIQUES FC, 1947, ARCH PATHOL, V43, P489
[10]  
JOINER CH, 1979, BIOCHIM BIOPHYS ACTA, V552, P540, DOI 10.1016/0005-2736(79)90199-8