INTRACELLULAR CALCIUM INCREASE INDUCED BY GABA IN VISUAL-CORTEX OF FETAL AND NEONATAL RATS AND ITS DISAPPEARANCE WITH DEVELOPMENT

被引:64
作者
LIN, MH [1 ]
TAKAHASHI, MP [1 ]
TAKAHASHI, Y [1 ]
TSUMOTO, T [1 ]
机构
[1] OSAKA UNIV,SCH MED,BIOMED RES CTR,DEPT NEUROPHYSIOL,SUITA,OSAKA 565,JAPAN
关键词
GABA; GABA RECEPTORS; CALCIUM; CALCIUM FLUOROMETRY; RHOD-2; VISUAL CORTEX; DEVELOPMENT; RAT;
D O I
10.1016/0168-0102(94)90025-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To address the question of whether gamma-aminobutyric acid (GABA) induces a change in the concentration of Ca2+ in neurons of the developing visual cortex, and if so, to elucidate a developmental profile of such a GABA-induced change, we measured intracellular Ca2+ signals using microscopic fluorometry in visual cortical slices loaded with rhod-2. The slices were prepared from rat fetuses of embryonic day 18 (E18) and rat pups of postnatal days 0-30 (P0-P30). Application of GABA through the perfusate at 100 mu M induced a marked rise in intracellular Ca2+ signals in the cortical plate and subplate at E18 and P0-P2. After P5 the GABA-induced rise in Ca2+ dramatically reduced, and at P20 and thereafter it became undetectable. At E18 and P0-P2 an agonist for GABA, receptor, muscimol, induced a Ca2+ rise in the same way as did GABA, while a GABA(B) receptor agonist, baclofen, did not induce any significant rise in Ca2+ signals. Also, a GABA(A) receptor antagonist, bicuculline, blocked the GABA-induced rise in Ca2+ signals. These results indicate that the Ca2+ rise is triggered by activation of GABA(A) receptors. The application of Ni2+ at a concentration high enough to block all types of voltage-dependent Ca2+ channels prevented the Ca2+ signals from increasing in response to GABA application, suggesting that Ca2+ may be influxed through such channels following depolarization evoked by GABA.
引用
收藏
页码:85 / 94
页数:10
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