Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology

A 17-run Box-Behnken design (BBD) was used to optimize the extraction conditions of astaxanthin from shrimp waste. Three factors such as ratio of ethanol to raw material, extraction temperature (degrees C) and extraction time (min) were investigated. The adjusted coefficient of determination (R-adj(2)) for the model was 0.9218, and the probability value (p=0.0003) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized ratio of ethanol to raw material 29.7, extraction temperature 49.5 degrees C and extraction time 59.9 min. Under these conditions, the mean extraction yield of astaxanthin was 17.80 mu g/g, which was in good agreement with the predicted model value. Under these conditions, validation experiments were done and the mean extraction yield of astaxanthin was 17.77 mu g/g, which is in good agreement with the predicted model value.