MUTANT OF THE GLUTAMINE TRANSFER-RNA GENE AS UGA SUPPRESSOR IN ESCHERICHIA-COLI

被引：4
|
作者：
INOKUCHI, H
KONDO, K
YOSHIMURA, M
OZEKI, H
机构：
[1] Department of Biophysics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto
来源：
MOLECULAR AND GENERAL GENETICS | 1990年 / 223卷 / 03期
关键词：
Escherichia coli; Lethal effect; tRNA[!sub]2[!/sub][!sup]Gln[!/sup]gene; UGA suppressor;
D O I：
10.1007/BF00264450
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A UGA suppressor derived from a glutamine tRNA gene of Escherichia coli K 12 was isolated and characterized. Phages carrying the suppressor su+2UGA could be obtained only from a hybrid transducing phage, h80cI857p su+2oc, but not from the original transducing phage λcI857p su+2oc. By DNA sequence analysis, it was found that the su+2 UGA suppressor obtained has two mutations; one is in the anticodon (TTA→TCA), as expected, and the other (C→T) is at the 7th position from the 3′ end of tRNA2Gln. The significance of these mutations and the lethal effect on phage λ of the increased amounts of UGA suppressor tRNAs are discussed. © 1990 Springer-Verlag.
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页码：433 / 437
页数：5
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