EXPOSURE OF MAMMALIAN-CELLS TO 60-HZ MAGNETIC OR ELECTRIC-FIELDS - ANALYSIS OF DNA-REPAIR OF INDUCED, SINGLE-STRAND BREAKS

DNA damage was induced in isolated human peripheral lymphocytes by exposure at 5 Gy to 60Co radiation. Cells were permitted to repair the DNA damage while exposed to 60‐Hz fields or while sham‐exposed. Exposed cells were subjected to magnetic (B) or electric (E) fields, alone or in combination, throughout their allotted repair time. Repair was stopped at specific times, and the cells were immediately lysed and then analyzed for the presence of DNA single‐strand breaks (SSB) by the alkaline‐elution technique. Fifty to 75 percent of the induced SSB were repaired 20 min after exposure, and most of the remaining damage was repaired after 180 min. Cells were exposed to a 60‐Hz ac B field of 1 mT; an E field of 1 or 20 V/m; or combined E and B fields of 0.2 V/m and 0.05 mT, 6 V/m and 0.6 mT, or 20 V/m and 1 mT. None of the exposures was observed to affect significantly the repair of DNA SSB. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company