Serum Hepcidin Evaluation in Patients with Chronic Dialysis

被引：2
作者：
Manolov, V. [1 ]
Yonova, D. [2 ]
Vazelov, E. [2 ]
Bogov, B. [3 ]
Velizarova, M. [1 ]
Atanasova, B. [4 ]
Vasilev, V. [4 ]
Tzatchev, K. [4 ]
Bogov, I. [5 ]
机构：
[1] Med Univ, Dept Med Genet, Sofia, Bulgaria
[2] Med Univ, Clin Ctr Dialysis, Sofia, Bulgaria
[3] Med Univ, Dept Clin Nephrol, Sofia, Bulgaria
[4] Med Univ, Dept Clin Lab & Clin Immunol, Sofia, Bulgaria
[5] Natl Cardiol Hosp, Sofia, Bulgaria
来源：
INTERNATIONAL JOURNAL OF BIOMEDICINE | 2015年 / 5卷 / 02期
关键词：
Hepcidin; anemia of chronic disease; chronic kidney disease (CKD); hemodialysis;
D O I：
10.21103/Article5(2)_CR5
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The aim of our study was to quantify serum hepcidin levels in Bulgarian patients with chronic kidney disease (CKD). Expected high values of peptide hormones might provide a new therapeutic choice for anemia of chronic disease. We looked for correlation between serum hepcidin levels and some iron metabolism parameters in CKD patients. Results: The sandwich ELISA is highly specific for hepcidin-25. We found statistically significant differences in serum hepcidin levels in patients of the control group, with CKD stages II to IV and CKD stage V (on chronic dialysis): 12.7+/-8.7 mu g/L, 90.74+/-21.1 mu g/L, and 282.49+/-81.1 mu g/L, respectively. Significant correlation between serum hepcidin and transferrin saturation (r=0.340, P<0.05) was found in the group of patients with CKD stage V. Conclusion: The use of 2 monoclonal antibodies in a sandwich ELISA format provides a reliable, reproducible, and inexpensive method for measuring serum concentrations of the bioactive form of hepcidin in Bulgarian laboratory practice.
引用
收藏
页码：76 / 78
页数：3
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