DNA OF ACANTHAMOEBA SPP - A METHOD FOR EXTRACTION AND ITS CHARACTERIZATION

被引：21
作者：
ADAM, KMG
BLEWETT, DA
FLAMM, WG
机构：
[1] Department of Zoology, University of Edinburgh
来源：
JOURNAL OF PROTOZOOLOGY | 1969年 / 16卷 / 01期
关键词：
D O I：
10.1111/j.1550-7408.1969.tb02225.x
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
SYNOPSIS. A method is described which permits the recovery of a high yield of undegraded DNA from Acanthamoeba castellanii. The DNA of A. castellanii is comprised of 2 species: the buoyant density of the major component is 1.720 and of the minor component, 1.693. The renaturation characteristics of the minor component suggest that it is of mitochondrial origin. Two components are also present in the DNA of A. palestinensis, A. polyphaga and A. astronyxis. The buoyant densities of major and minor components differ between species. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码：6 / &
相关论文
共 47 条
[11]   AN IMPROVED DIPHENYLAMINE METHOD FOR ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].
GILES, KW ;
MYERS, A .
NATURE, 1965, 206 (4979) :93-&
[12]   SATELLITE DNAS OF SOME HIGHER PLANTS [J].
GREEN, BR ;
GORDON, MP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 145 (02) :378-&
[13]   MITOCHONDRIAL DNA SYNTHESIS AND MITOTIC CYCLE IN PHYSARUM POLYCEPHALUM [J].
GUTTES, EW ;
HANAWALT, PC ;
GUTTES, S .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 142 (01) :181-&
[14]   DETERMINATION OF DENSITY DISTRIBUTIONS AND DENSITY GRADIENTS IN BINARY SOLUTIONS AT EQUILIBRIUM IN ULTRACENTRIFUGE [J].
IFFT, JB ;
VOET, DH ;
VINOGRAD, J .
JOURNAL OF PHYSICAL CHEMISTRY, 1961, 65 (07) :1138-&
[15]  
KIDDER GW, 1967, CHEMICAL ZOOLOGY ED, V1, P541
[16]   NEW METHOD FOR THE ISOLATION OF DEOXYRIBONUCLEIC ACIDS - EVIDENCE ON THE NATURE OF BONDS BETWEEN DEOXYRIBONUCLEIC ACID AND PROTEIN [J].
KIRBY, KS .
BIOCHEMICAL JOURNAL, 1957, 66 (03) :495-504
[17]  
KIRBY KS, 1962, BIOCHIM BIOPHYS ACTA, V55, P382
[18]  
KIRBY KS, 1962, BIOCHIM BIOPHYS ACTA, V55, P545
[19]  
KIRTIKAR M, 1967, J PROTOZOOL, VS 14, P11
[20]   DNA SATELLITES FROM CELLS OF GREEN AND APLASTIDIC ALGAE [J].
LEFF, J ;
MANDEL, M ;
EPSTEIN, HT ;
SCHIFF, JA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1963, 13 (02) :126-&
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