SIMULTANEOUS DETERMINATION OF CATECHOLAMINES, THEIR BASIC METABOLITES AND SEROTONIN IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A MIXED-MODE COLUMN AND AN 8-CHANNEL ELECTROCHEMICAL DETECTOR

被引：46

作者：

MASHIGE, F

MATSUSHIMA, Y

MIYATA, C

YAMADA, R

KANAZAWA, H

SAKUMA, I

TAKAI, N

SHINOZUKA, N

OHKUBO, A

NAKAHARA, K

机构：

[1] KYORITSU COLL PHARMACEUT SCI,MINATO KU,TOKYO 105,JAPAN

[2] TOKYO DENKI UNIV,FAC SCI & ENGN,HATOYAMA,SAITAMA 35003,JAPAN

[3] TOKYO MED COLL,SHINJUKU KU,TOKYO 160,JAPAN

[4] UNIV TOKYO,INST IND SCI,MINATO KU,TOKYO 106,JAPAN

来源：

BIOMEDICAL CHROMATOGRAPHY | 1995年 / 9卷 / 05期

关键词：

D O I：

10.1002/bmc.1130090506

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation exchange resin and a mixed-mode (C-18/cation-exchange) column and determined by an eight-channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3-methoxy-tyramine (3-MT) and serotonin (5-HT) were determined in 40 min with inter-assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3-MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3-MT, 5-HT were 99, 104, 104, 99, 102, 109 and 99%, respectively.

引用

页码：221 / 225

页数：5

共 14 条

[11]

SAKUMA I, 1986, PRECISION MACH, V1, P231

[12]

STROBEL G, 1991, CLIN CHEM, V37, P196

[13]

TAKAI N, 1992, PROGR CLIN BIOCH, P157

[14] DETERMINATION OF URINARY NORMETANEPHRINE, METANEPHRINE AND 3-METHOXYTYRAMINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - COMPARISON BETWEEN AUTOMATED COLUMN-SWITCHING AND MANUAL DUAL-COLUMN SAMPLE PURIFICATION METHODS [J].

VOLIN, P .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 578 (02) :165-174

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