PARTIAL-PURIFICATION OF TEMPLATE-ACTIVE FRACTION OF CHROMATIN - PRELIMINARY REPORT

被引：232

作者：

GOTTESFELD, JM ^{[1
]}

GARRARD, WT ^{[1
]}

BAGI, G ^{[1
]}

WILSON, RF ^{[1
]}

BONNER, J ^{[1
]}

机构：

[1] CALTECH, DIV BIOL, PASADENA, CA 91109 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1974年 / 71卷 / 06期

关键词：

D O I：

10.1073/pnas.71.6.2193

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A fraction of rat liver chromatin that is transcriptionally active in vivo has been purified 6- to 7 fold over whole chromatin. This was accomplished by selectively shearing chromatin with DNase II followed by fractionating the released portion on the basis of its solubility properties in 2 mM MgCl 2. The resulting soluble material comprises 11% of the total chromatin DNA and is impoverished in histone and enriched in nonhistone protein. Compared with unsheared chromatin, this minor fraction exhibits marked differences in chromosomal protein species. DNA renaturation studies indicate that this fraction is composed of a specific subset of whole genomal DNA sequences. Furthermore, DNA - RNA hybridization experiments suggest that almost 60% of the nonrepetitious DNA sequences of this minor fraction could code for cellular RNA.

引用

页码：2193 / 2197

页数：5

共 28 条

[11]

GARRARD WT, IN PRESS

[12] TISSUE-SPECIFIC TRANSCRIPTION OF GLOBIN GENE IN ISOLATED CHROMATIN [J].