ENDOGLUCANASE CASA FROM ALKALOPHILIC STREPTOMYCES STRAIN KSM-9 IS A TYPICAL MEMBER OF FAMILY-B OF BETA-1,4-GLUCANASES

被引：14

作者：

DAMUDE, HG ^{[1
]}

GILKES, NR ^{[1
]}

KILBURN, DG ^{[1
]}

MILLER, RC ^{[1
]}

ANTONY, R ^{[1
]}

WARREN, J ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT MICROBIOL,300-6174 UNIV BLVD,VANCOUVER V6T 1Z3,BC,CANADA

来源：

GENE | 1993年 / 123卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CATALYTIC SITE; CELLOBIOHYDROLASE; CELLULASE; FUNCTIONAL DOMAINS; DISULFIDES; SEQUENCE HOMOLOGY; STEREOCHEMISTRY;

D O I：

10.1016/0378-1119(93)90547-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

CasA is an endo-beta-1,4-glucanase from Streptomyces KSM-9 belonging to family B of beta-1,4-glucanases. A previous analysis of a portion of the corresponding gene (casA) revealed sequencing errors in a region encoding part of the catalytic site. Additional errors in the original sequence were suspected, based on sequence comparison of the C terminus of CasA with other members of its family. Re-sequencing of the remainder of the casA coding region showed that CasA is a typical member of family B.

引用

页码：105 / 107

页数：3

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