CLONING AND CHARACTERIZATION OF THE AROA GENE FROM MYCOBACTERIUM-TUBERCULOSIS

被引：32

作者：

GARBE, T ^{[1
]}

JONES, C ^{[1
]}

CHARLES, I ^{[1
]}

DOUGAN, G ^{[1
]}

YOUNG, D ^{[1
]}

机构：

[1] WELLCOME RES LABS, BIOTECH, DEPT MOLEC BIOL, BECKENHAM BR3 3BS, KENT, ENGLAND

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 12期

关键词：

D O I：

10.1128/jb.172.12.6774-6782.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the λgt11 vector. Detailed characterization of the M. tuberculosis aroA gene by nucleotide sequencing and by immunochemical analysis of the expressed product indicates that it encodes a 5-enolpyruvylshikimate-3-phosphate synthase that is structurally related to analogous enzymes from other bacterial, fungal, and plant sources. The potential use of the cloned genes in construction of genetically defined mutant strains of M. tuberculosis by gene replacement is proposed as a novel approach to the rational attenuation of mycobacterial pathogens and the possible development of new antimycobacterial vaccines.

引用

页码：6774 / 6782

页数：9

共 38 条

[1]

[Anonymous], 1986, INFECT IMMUN, V51, P718

[2] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[3]

BROSIUS J, 1982, J BIOL CHEM, V257, P9205

[4] TUBERCULOSIS AND HUMAN IMMUNODEFICIENCY VIRUS-INFECTION [J].

CHAISSON, RE ;

SLUTKIN, G .

JOURNAL OF INFECTIOUS DISEASES, 1989, 159 (01) :96-100

[5] THE ISOLATION AND NUCLEOTIDE-SEQUENCE OF THE COMPLEX AROM LOCUS OF ASPERGILLUS-NIDULANS [J].

CHARLES, IG ;

KEYTE, JW ;

BRAMMAR, WJ ;

SMITH, M ;

HAWKINS, AR .

NUCLEIC ACIDS RESEARCH, 1986, 14 (05) :2201-2213

[6] MOLECULAR ANALYSIS OF DNA AND CONSTRUCTION OF GENOMIC LIBRARIES OF MYCOBACTERIUM-LEPRAE [J].

CLARKCURTISS, JE ;

JACOBS, WR ;

DOCHERTY, MA ;

RITCHIE, LR ;

CURTISS, R .

JOURNAL OF BACTERIOLOGY, 1985, 161 (03) :1093-1102

[7] VACCINES AND CELL-MEDIATED-IMMUNITY [J].

COLLINS, FM .

BACTERIOLOGICAL REVIEWS, 1974, 38 (04) :371-402

[8]

Davis RW, 1980, ADV BACTERIAL GENETI

[9] THE COMPLETE AMINO-ACID-SEQUENCE OF ESCHERICHIA-COLI 5-ENOLPYRUVYLSHIKIMATE 3-PHOSPHATE SYNTHASE [J].

DUNCAN, K ;

LEWENDON, A ;

COGGINS, JR .

FEBS LETTERS, 1984, 170 (01) :59-63

[10]

ENGERS HD, 1985, INFECT IMMUN, V48, P603

← 1 2 3 4 →