CORONARY REPERFUSION IN DOGS INHIBITS ENDOTHELIUM-DEPENDENT RELAXATION - ROLE OF SUPEROXIDE RADICALS

被引：32

作者：

LAWSON, DL ^{[1
]}

MEHTA, JL ^{[1
]}

NICHOLS, WW ^{[1
]}

机构：

[1] UNIV FLORIDA,J HILLIS MILLER HLTH CTR,COLL MED,DEPT MED,BOX J-277,GAINESVILLE,FL 32610

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 04期

关键词：

Endothelium-dependent coronary relaxation; Free radicals; Superoxide dismutase; Superoxide radicals; Xanthine; Xanthine oxidase;

D O I：

10.1016/0891-5849(90)90103-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies indicate that release of superoxide radicals during coronary reperfusion following occlusion may relate to the loss of endothelium-dependent coronary arterial relaxation. We examined coronary arterial ring relaxation in dogs subjected to temporary circumflex (Cx) coronary artery occlusion and treated with saline or the superoxide radical scavenger superoxide dismutase (SOD). In dogs treated with saline, Cx coronary ring relaxation in response to leukotriene D4 (LTD4) and acetylcholine (ACh) was attenuated (p < 0.01), but coronary relaxation in response to nitroglycerin was preserved, suggesting loss of endothelium-dependent relaxation following coronary reperfusion. In contrast, Cx coronary relaxation in response to LTD4 and ACh was preserved in the SOD-treated dogs (p < 0.01 compared to saline-treated dogs). To further examine the role of superoxide radicals in the loss of endothelium-dependent relaxation, normal nonischemic canine coronary artery and rat aortic rings were exposed to a superoxide radical generating system of xanthine and xanthine oxidase in vitro. Xanthine plus xanthine oxidase treatment caused a significant (p < 0.01) decrease in the relaxant effects of ACh. Pretreatment of rat aortic rings with SOD protected against the loss of ACh-induced relaxation. These observations suggest that release of superoxide radicals during reperfusion is the basis of loss of endothelium-dependent coronary arterial relaxation. Treatment with superoxide radical scavengers prior to coronary reperfusion protects against this loss. © 1990.

引用

页码：373 / 380

页数：8

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