Chemiluminometric flow-injection method for determination of free L-malate in wine with co-immobilized malate dehydrogenase NADH oxidase

被引：13
作者：
Kiba, N
Inagaki, J
Furusawa, M
机构：
[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu
来源：
TALANTA | 1995年 / 42卷 / 11期
关键词：
chemiluminescence; flow injection; co-immobilized enzyme reactor; malic acid wine;
D O I：
10.1016/0039-9140(95)01660-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow-injection system with a co-immobilized malate dehydrogenase/reduced nicotineamide adenine dinucleotide (NADH) oxidase reactor and a chemiluminometer is described for the determination of free L-malate in wine. Malate dehydrogenase and NADH oxidase were co-immobilized on poly(vinyl alcohol) beads and packed into a stainless-steel column (5 cm x 4 mm i.d.). The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate(III) reaction. The calibration graph was linear from 3 x 10(-7) M to 2.5 x 10(-4) M (the linear correlation coefficient was 0.9998); the detection limit (signal-to-noise ratio, 3) was 8 x 10(-8) M. The sample throughput was 30 h(-1) without carryover. The reactor was renewed every 2 weeks.
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页码：1751 / 1755
页数：5
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