PURIFICATION AND CHARACTERIZATION OF LIVER ARYLFORMAMIDASE IN RAINBOW-TROUT AND CATTLE

被引：2
|
作者：
SERRANO, AE
NAGAYAMA, F
机构：
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 98卷 / 2-3期
关键词：
D O I：
10.1016/0305-0491(91)90194-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. Arylformamidases were purified from the liver of rainbow trout and cattle. 2. Optimal pH's were 7.7 and 8.0 for the fish and cattle enzyme, respectively. Both enzymes showed optimum temperature at 40-degrees-C. Thermal and pH stability ranges and Arrhenius plots of the enzymes differed. 3. K(m) and molecular weight of the fish enzyme were determined to be 0.28 mM and 32,700, respectively, while those for the cattle enzyme were 0.78 mM and 42,5000, respectively.
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页码：381 / 387
页数：7
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