VISIBLE-LIGHT EFFECTS ON TUMORAL CELLS IN A CULTURE-MEDIUM ENRICHED WITH TRYPTOPHAN AND RIBOFLAVIN

被引:64
作者
EDWARDS, AM
SILVA, E
JOFRE, B
BECKER, MI
DEIOANNES, AE
机构
[1] PONTIFICIA UNIV CATOLICA CHILE, FAC QUIM 536, SANTIAGO 22, CHILE
[2] PONTIFICIA UNIV CATOLICA CHILE, FAC CIENCIAS BIOL, SANTIAGO, CHILE
关键词
CYTOTOXICITY; TUMORAL CELLS; RIBOFLAVIN; TRYPTOPHAN; IRRADIATION; OXYGEN REACTIVE SPECIES; TRANSMISSION ELECTRON MICROSCOPY; APOPTOSIS;
D O I
10.1016/1011-1344(94)07020-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When NSO/2 myeloid cell line and teratocarcinoma F9 cells were irradiated in Dulbecco's modified Eagle medium enriched with tryptophan and riboflavin, toxic photoproducts for these tumoral cells were generated. The active participation of O-1(2) and .OH was established using specific scavengers and quenchers. A cytotoxic effect was also observed when unirradiated tumoral cells were incubated in a previously irradiated culture medium enriched with tryptophan and riboflavin. When irradiated medium was used alone, enriched only with tryptophan or only with riboflavin, no toxic effect was observed. The relevance of charge transfer processes between triplet riboflavin and tryptophan in the generation of cytotoxic photoproducts is discussed.
引用
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页码:179 / 186
页数:8
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