A NEW SOURCE OF CYTOPLASMIC MALE-STERILITY IN PEARL-MILLET - RFLP ANALYSIS OF MITOCHONDRIAL-DNA

被引：11

作者：

SUJATA, V ^{[1
]}

SIVARAMAKRISHNAN, S ^{[1
]}

RAI, KN ^{[1
]}

SEETHA, K ^{[1
]}

机构：

[1] INT CROPS RES INST SEMI ARID TROP,PATANCHERU 502324,ANDHRA PRADESH,INDIA

来源：

GENOME | 1994年 / 37卷 / 03期

关键词：

PENNISETUM GLAUCUM; CYTOPLASMIC MALE STERILITY; MITOCHONDRIAL DNA; RFLP;

D O I：

10.1139/g94-067

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new source of cytoplasmic male sterility (ems) in pearl millet (Pennisetum glaucum (L.) R.Br.) derived from a half-sib progeny of the Early Gene Pool (EGP 261) and used in a male-sterile line, ICMA 90111, was compared with other known ems sources for RFLP of mitochondrial (mt) DNA. Southern blot hybridization of mtDNA from ICMA 90111 digested with several restriction enzymes and probed with homologous mtDNA clones from pearl millet and heterologous gene clones from maize and wheat revealed the RFLP patterns of ICMA 90111 distinct from others studied so far. The dendrogram of male-sterile lines constructed from the Southern blot hybridization patterns indicated that ICMA 90111 represents a separate group. Our results suggest that this source of ems is unique in several respects.

引用

页码：482 / 486

页数：5

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