THE MURINE GENE ENCODING THE HIGHLY CONSERVED SM-B PROTEIN CONTAINS A NONFUNCTIONAL ALTERNATIVE 3' SPLICE SITE

被引：12

作者：

GRIFFITH, AJ ^{[1
]}

SCHMAUSS, C ^{[1
]}

CRAFT, J ^{[1
]}

机构：

[1] YALE UNIV, SCH MED, 333 CEDAR ST, POB 3333, 610 LCI, NEW HAVEN, CT 06510 USA

来源：

GENE | 1992年 / 114卷 / 02期

关键词：

ALTERNATIVE SPLICING; AUTOANTIBODIES; SMALL NUCLEAR RIBONUCLEOPROTEIN; SYSTEMIC LUPUS ERYTHEMATOSUS;

D O I：

10.1016/0378-1119(92)90574-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNA and partial genomic nucleotide (nt) sequences were derived for the mouse Sm B polypeptide and compared to the cDNA and genomic sequences encoding human Sm B. The deduced amino acid (aa) sequences from the mouse and human genes are identical with the exception of a single conserved aa substitution, accounting for the ability of anti-Sm antibodies to recognize the Sm polypeptides from a broad range of species. The genomic sequence of mouse B gene is similar to the human B genomic locus that extends from exon 6 to exon 7. These loci include conservation of both 3' alternative splice sites and putative branch points required to process B and B' mRNAs in human cells. However, the nt sequence downstream from the putative distal 3' splice junction and single nt flanking the 3' splice site consensus sequence, differ between mouse and human B. This results in a murine mRNA with a different predicted secondary structure around the distal 3' splice site when compared to humans. Thus, secondary structural constraints in the mRNA or changes in the exon sequence might prevent recognition of this alternative splice site to form B' mRNA in murine tissues.

引用

页码：195 / 201

页数：7

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