In vitro meristem culture of juvenile and mature Sequoiadendron giganteum

被引：20
作者：
Monteuuis, O. [1 ]
机构：
[1] AFOCEL, F-77370 Domaine De Letancon, Nangis, France
来源：
TREE PHYSIOLOGY | 1987年 / 3卷 / 03期
关键词：
D O I：
10.1093/treephys/3.3.265
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
A total of 7000 meristems were used in experiments to investigate the possibility of cloning Sequoiadendron giganteum Buchholz by in vitro meristem Culture of juvenile (2-year-old) and mature (100-year-old) ortets, Cultures were initiated on a low-salt medium containing 0.1 mg l(-1) 1-naphthaleneacetic acid to stimulate meristematic activity. Benzylamino purine (0.01-0.5 mg l(-1)) inhibited meristematic activity, whereas gibberellic acid (0.01-0.5 mg l(-1)) had no effect on meristematic development. The mature ortet showed more specific mineral requirements and a lower capacity for cloning than the juvenile ortet. Rooted plants were obtained only from the juvenile clone, There was a marked seasonal effect on meristematic activity, especially for the mature clone, the most active material being obtained during budbreak.
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页码：265 / 272
页数：8
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