CATALYTIC AND ACTIVATING PROTONS FOLLOW DIFFERENT PATHWAYS IN THE H+-ATPASE OF POTATO-TUBER MITOCHONDRIA

被引：6
|
作者：
VALERIO, M
HARAUX, F
机构：
[1] Biosystèmes Membranaires (ERS 30), CNRS
来源：
FEBS LETTERS | 1993年 / 336卷 / 01期
关键词：
F0F1; H+-ATPASE; ELECTROCHEMICAL PROTON GRADIENT; ENZYME ACTIVATION; INHIBITOR; LAURYLDIMETHYLAMINE OXIDE; PLANT MITOCHONDRIA;
D O I：
10.1016/0014-5793(93)81614-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of some F0F1 inhibitors on the activation of the H+-ATPase by the electrochemical proton gradient was investigated in mitochondria extracted from potato tubers. Transient activated state of the ATPase was revealed by addition of ATP and of the detergent lauryldimethylamine oxide (LDAO) to energized mitochondria. Venturicidin, tri-n-butyltin and aurovertin at high concentrations did not affect the process of Delta<(mu)over tilde>(+)(H)-activation, whereas oligomycin fully blocked it. The results support the idea of separate pathways or binding sites for catalytic and activating protons.
引用
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页码：83 / 86
页数：4
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