RESISTANCE TO LATE BLIGHT (PHYTOPHTHORA-INFESTANS (MONT) DEBARY) OF POTATO PLANTS REGENERATED FROM IN-VITRO SELECTED CALLI

被引:6
作者
CERATO, C [1 ]
MANICI, LM [1 ]
BORGATTI, S [1 ]
ALICCHIO, R [1 ]
GHEDINI, R [1 ]
GHINELLI, A [1 ]
机构
[1] UNIV BOLOGNA,DEPT BIOL,I-40126 BOLOGNA,ITALY
关键词
CULTURE FILTRATE; RESISTANT VARIANTS; SOLANUM-TUBEROSUM L;
D O I
10.1007/BF02361801
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Cells of seven potato cultivars were selected in vitro with culture filtrate (CF) of Phytophthora infestans (Mont.) de Bary. Regenerated plants were tested for late blight resistance. The aim of the study was to check the efficacy of CF in the selection of potatoes for resistance to late blight and to evaluate the effects of additive factors on general resistance. One selection cycle, applied to a cell culture system, selected cells resistant to toxic metabolites of CF. In vivo screening of clones regenerated from selected cells was done in two steps: on whole plants, assessing foliage late blight, and on detached leaves, assessing single factors of horizontal resistance. In general, the frequency of resistant variants selected with CF did not differ from that of resistant somaclonal variants. Nevertheless, high concentrations of CF in the growth medium seemed to induce an improvement of some partial resistance factors as compared with the source plants.
引用
收藏
页码:341 / 351
页数:11
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