HYPOTHALAMIC CATHEPSIN-D - ASSAY AND ISOENZYME COMPOSITION

被引：10

作者：

AKOPYAN, TN ^{[1
]}

BARCHUDARYAN, NA ^{[1
]}

KARABASHYAN, LV ^{[1
]}

ARUTUNYAN, AA ^{[1
]}

LAJTHA, A ^{[1
]}

GALOYAN, AA ^{[1
]}

机构：

[1] CTR NEUROCHEM,WARDS ISL,NY

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1979年 / 4卷 / 5-6期

关键词：

acid proteinases; cathepsin D; endopeptidase assay;

D O I：

10.1002/jnr.490040505

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A sensitive and convenient method of endopeptidase assay using as substrate globin modified with pyridoxal‐5‐phosphate was used for determination of acid proteinases in bovine hypothalamus separated by isoelectric focusing. The soluble protein fraction of hypothalamus upon elution from Sephadex gave five peaks of proteinase activity at pH 3.2. The properties indicate that these peaks of endopeptidase activity are isoenzyme forms of cathepsin D. Copyright © 1979 Alan R. Liss, Inc.

引用

页码：365 / 370

页数：6

共 19 条

[1] ACID PROTEINASE OF HYPOTHALAMUS PURIFICATION, SOME PROPERTIES, AND ACTION ON SOMATOSTATIN AND SUBSTANCE-P [J].