STABILIZING FACTOR(S) OF NITRIC-OXIDE (NO) SYNTHETASE

被引：9
作者：
KOSUGA, K
YUI, Y
HATTORI, R
EIZAWA, H
HIKI, K
KAWAI, C
机构：
[1] Third Division, Department of Internal Medicine, Faculty of Medicine, Kyoto
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90731-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We found that the cytosol of rat peritoneal polymorphonuclear neutrophils contains factor(s) that can stabilize an unstable enzyme, nitric oxide synthetase, in the cytosol. This enzyme has been purified to a single protein from the cytosol. Its half-life was 3 hours at 4°C and was prolonged to >24 hours by the stabilizing factor in the cytosol. The molecular weight of the stabilizing factor was >100,000. Its activity was lost by the treatment with heating or alkali for 1 min or with acid for 5 min. It did not adhere to the carboxymethyl or diethylaminoethyl column at neutral pH. This stabilizing factor(s) may play a role in the regulation of the nitric oxide synthetase. © 1990.
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页码：705 / 708
页数：4
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