DIRECT LYTIC FACTOR OF COBRA VENOM - PURIFICATION AND CHEMICAL CHARACTERIZATION

被引：75
作者：
ALOOFHIRSCH, S
DEVRIES, A
BERGER, A
机构：
[1] The Rogoff-Wellcome Medical Research Institute, Tel Aviv University, Beilinson Medical Center, Petah-Tikva
[2] Department of Biophysics, The Weizmann Institute of Science, Rehovoth
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 154卷 / 01期
关键词：
D O I：
10.1016/0005-2795(68)90257-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The direct lytic factor (DLF) from the venom of Ringhals (Haemachatus haemachates) was isolated and purified by successive application of trichloroacetic acid and salt precipitation. The purified DLF is homogeneous by ultracentrifugal and electrophoretic criteria and is about twice as active as the material isolated previously by paper electrophoresis. DLF is a single-chain protein with a molecular weight of 7000. It contains 57 amino acid residues cross-linked intramolecularly by 4 disulfide bridges. Leucine is in the amino terminal position, and serine in the carboxyl terminal position of the protein. DLF causes inhibition of growth in some bacteria. © 1968.
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页码：53 / +
页数：1
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