MOLECULAR-WEIGHTS OF HIGH-MOLECULAR-WEIGHT SUBUNITS OF GLUTENIN DETERMINED BY MASS-SPECTROMETRY

被引：53

作者：

HICKMAN, DR ^{[1
]}

ROEPSTORFF, P ^{[1
]}

SHEWRY, PR ^{[1
]}

TATHAM, AS ^{[1
]}

机构：

[1] ODENSE UNIV,DEPT MOLEC BIOL,DK-5230 ODENSE M,DENMARK

来源：

JOURNAL OF CEREAL SCIENCE | 1995年 / 22卷 / 02期

关键词：

GLUTENIN SUBUNITS; MOLECULAR WEIGHTS; MASS SPECTROMETRY;

D O I：

10.1016/0733-5210(95)90038-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

High molecular weight (HMW) subunits of glutenin, corresponding to those for which full length gene sequences are available, were purified and their M(r)s determined by mass spectrometry [matrix-assisted laser desorption ionisation (MALDI)]. In general, the M(r)s determined were within the experimental error of the techniques employed [at the mass range of the high molecular weight (HMW) prolamins, error similar to 500 Da]. Two subunits, 1Dx2 and 1By9, however, showed M(r)s approximately 900 Da higher and 1700 Da lower, respectively, than expected from their gene sequences. These data confirm that the HMW subunits are not extensively glycosylated and also demonstrate the utility of mass spectrometry for determining the M(r)s of prolamins. (C) 1995 Academic Press Limited

引用

页码：99 / 103

页数：5

共 24 条

[1] THE CHARACTERIZATION AND COMPARATIVE-ANALYSIS OF HIGH-MOLECULAR-WEIGHT GLUTENIN GENES FROM GENOME-A AND GENOME-B OF A HEXAPLOID BREAD WHEAT [J].