CHARACTERIZATION OF THE PET OPERON OF RHODOSPIRILLUM-RUBRUM

被引:5
作者
SHANKER, S
MOOMAW, C
GUNER, S
HSU, J
TOKITO, MK
DALDAL, F
KNAFF, DB
HARMAN, JG
机构
[1] TEXAS TECH UNIV,DEPT CHEM & BIOCHEM,LUBBOCK,TX 79409
[2] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,RES LABS,DALLAS,TX 75235
[3] KARADENIZ TECH UNIV,DEPT CHEM,TRABZON,TURKEY
[4] UNIV PENN,INST PLANT SCI,DEPT BIOL,PHILADELPHIA,PA 19104
关键词
D O I
10.1007/BF00035943
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The three genes of the pet operon, coding, respectively, for the Rieske iron-sulfur protein, cytochrome b and cytochrome c1 components of the cytochrome bc1 complex in the photosynthetic bacterium Rhodospirillum rubrum have been sequenced. The amino acid sequences deduced for these three peptides from the nucleotide sequences of the genes have been confirmed, in part, by direct sequencing of portions of the three peptides separated from a sample of the purified, detergent-solubilized complex. These sequences show considerable homology with those previously obtained for the pet operons of other photosynthetic bacteria. Northern blots of R. rubrum mRNA have established that the operon is transcribed as a single polycistronic message, the start site of which has been determined by both primer extension and nuclease protection. Photosynthetic growth of R. rubrum was shown to be inhibited by antimycin A, a specific inhibitor of cytochrome bc1 complexes, and antimycin A-resistant mutants of R. rubrum have been isolated. Preliminary results suggest that it may be possible to express the R. rubrum pet operon in a strain of the photosynthetic bacterium Rhodobacter capsulatus from which the native pet operon has been deleted.
引用
收藏
页码:79 / 94
页数:16
相关论文
共 66 条
[1]   INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].
AEBERSOLD, RH ;
LEAVITT, J ;
SAAVEDRA, RA ;
HOOD, LE ;
KENT, SBH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :6970-6974
[2]   LARGE-SCALE PURIFICATION AND CHARACTERIZATION OF A HIGHLY-ACTIVE 4-SUBUNIT CYTOCHROME BC1 COMPLEX FROM RHODOBACTER-SPHAEROIDES [J].
ANDREWS, KM ;
CROFTS, AR ;
GENNIS, RB .
BIOCHEMISTRY, 1990, 29 (11) :2645-2651
[3]   SIZE OF THE AMINO-ACID SIDE-CHAIN AT POSITION-158 OF CYTOCHROME-B IS CRITICAL FOR AN ACTIVE CYTOCHROME-BC1 COMPLEX AND FOR PHOTOSYNTHETIC GROWTH OF RHODOBACTER-CAPSULATUS [J].
ATTAASAFOADJEI, E ;
DALDAL, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :492-496
[4]  
BENNOUN P, 1991, GENETICS, V127, P335
[5]   ELECTRON-SPIN ECHO ENVELOPE MODULATION SPECTROSCOPY SUPPORTS THE SUGGESTED COORDINATION OF 2 HISTIDINE LIGANDS TO THE RIESKE FE-S CENTERS OF THE CYTOCHROME-B6F COMPLEX OF SPINACH AND THE CYTOCHROME-BC1 COMPLEXES OF RHODOSPIRILLUM-RUBRUM, RHODOBACTER-SPHAEROIDES R-26, AND BOVINE HEART-MITOCHONDRIA [J].
BRITT, RD ;
SAUER, K ;
KLEIN, MP ;
KNAFF, DB ;
KRIAUCIUNAS, A ;
YU, CA ;
YU, L ;
MALKIN, R .
BIOCHEMISTRY, 1991, 30 (07) :1892-1901
[6]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[7]  
CULLY M, 1989, TECHNIQUES NEW DEV P, P287
[8]   MUTATIONS CONFERRING RESISTANCE TO QUINOL OXIDATION (QZ) INHIBITORS OF THE CYT-BC1 COMPLEX OF RHODOBACTER-CAPSULATUS [J].
DALDAL, F ;
TOKITO, MK ;
DAVIDSON, E ;
FAHAM, M .
EMBO JOURNAL, 1989, 8 (13) :3951-3961
[9]   ISOLATION OF THE STRUCTURAL GENES FOR THE RIESKE FE-S PROTEIN, CYTOCHROME-B AND CYTOCHROME-C1 ALL COMPONENTS OF THE UBIQUINOL - CYTOCHROME-C2 OXIDOREDUCTASE COMPLEX OF RHODOPSEUDOMONAS-CAPSULATA [J].
DALDAL, F ;
DAVIDSON, E ;
CHENG, S .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (01) :1-12
[10]   PRIMARY STRUCTURE OF THE BC1 COMPLEX OF RHODOPSEUDOMONAS-CAPSULATA - NUCLEOTIDE-SEQUENCE OF THE PET OPERON ENCODING THE RIESKE CYTOCHROME-B, AND CYTOCHROME-C1 APOPROTEINS [J].
DAVIDSON, E ;
DALDAL, F .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (01) :13-24