MEASUREMENTS OF LOCAL PH CHANGES NEAR BILAYER LIPID-MEMBRANE BY MEANS OF A PH MICROELECTRODE AND A PROTONOPHORE-DEPENDENT MEMBRANE-POTENTIAL - COMPARISON OF THE METHODS

被引：45
作者：
ANTONENKO, YN
BULYCHEV, AA
机构：
[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow
[2] Biophysical Department, Moscow State University, Moscow
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1070卷 / 01期
关键词：
BILAYER LIPID MEMBRANE; PH MICROELECTRODE; PH CHANGE; PROTONOPHORE; MEMBRANE POTENTIAL;
D O I：
10.1016/0005-2736(91)90176-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Shifts of pH near the bilayer lipid membrane (BLM) were measured in the absence of pH difference between bulk solutions by two methods, i.e. pH microelectrode and membrane potential recordings in the presence of a protonophore. A quantitative agreement of the results of both methods was obtained. The kinetics of the generation of potential induced by the addition of ammonium chloride was accounted for by the time of the diffusion through the unstirred layers. The thickness of the unstirred BLM layers was determined in the experiment.
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页码：279 / 282
页数：4
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