USE OF NITROCELLULOSE MEMBRANE TO ACTIVATE AND MEASURE INSECT PROPHENOL OXIDASE

被引：8
作者：
BREY, PT
STOLTZ, DB
COOK, DI
ASHIDA, M
机构：
[1] HOKKAIDO UNIV,INST LOW TEMP SCI,BIOCHEM LAB,SAPPORO,HOKKAIDO 060,JAPAN
[2] DALHOUSIE UNIV,DEPT MICROBIOL,HALIFAX B3H 4H7,NS,CANADA
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 02期
基金：
日本学术振兴会;
关键词：
D O I：
10.1016/0003-2697(91)90241-K
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator. © 1991.
引用
收藏
页码：359 / 364
页数：6
相关论文
共 30 条
[1]   ACTIVATION OF PRO-PHENOLOXIDASE BY THE ACTIVATING ENZYME OF THE SILKWORM, BOMBYX-MORI [J].
ASHIDA, M ;
DOHKE, K .
INSECT BIOCHEMISTRY, 1980, 10 (01) :37-+
[2]   PURIFICATION AND CHARACTERIZATION OF PRE-PHENOLOXIDASE FROM HEMOLYMPH OF SILKWORM BOMBYX-MORI [J].
ASHIDA, M .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 144 (02) :749-+
[3]   STUDIES ON PROPHENOLOXIDASE ACTIVATION IN THE MOSQUITO AEDES-AEGYPTI L [J].
ASHIDA, M ;
KINOSHITA, K ;
BREY, PT .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 188 (03) :507-515
[4]   LIMITED PROTEOLYSIS OF PROPHENOLOXIDASE DURING ACTIVATION BY MICROBIAL PRODUCTS IN INSECT PLASMA AND EFFECT OF PHENOLOXIDASE ON ELECTROPHORETIC MOBILITIES OF PLASMA-PROTEINS [J].
ASHIDA, M ;
YOSHIDA, H .
INSECT BIOCHEMISTRY, 1988, 18 (01) :11-+
[5]  
ASHIDA M, 1990, MOLTING METAMORPHOSI, P236
[6]  
ASHIDA M, 1982, 3RD P INT C INV PATH, P81
[7]   CHARACTERIZATION OF HEMOLYMPH PROTYROSINASE AND A CUTICULAR ACTIVATOR FROM MANDUCA-SEXTA (L) [J].
ASO, Y ;
KRAMER, KJ ;
HOPKINS, TL ;
LOOKHART, GL .
INSECT BIOCHEMISTRY, 1985, 15 (01) :9-+
[8]   IMMUNE RECOGNITION IN INSECTS - CONFLICTING EFFECTS OF AUTOLOGOUS PLASMA AND SERUM [J].
BREHELIN, M ;
BOEMARE, N .
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 1988, 157 (06) :759-764
[9]   STUDIES ON THE ACTIVATION OF THE PROPHENOLOXIDASE SYSTEM OF INSECTS BY BACTERIAL-CELL WALL COMPONENTS [J].
BROOKMAN, JL ;
RATCLIFFE, NA ;
ROWLEY, AF .
INSECT BIOCHEMISTRY, 1989, 19 (01) :47-57
[10]   THE METABOLISM OF THE AROMATIC AMINO-ACIDS CONCERNED IN THE CROSS-LINKING OF INSECT CUTICLE [J].
BRUNET, PCJ .
INSECT BIOCHEMISTRY, 1980, 10 (05) :467-500
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