RAPID SUBCHROMOSOMAL LOCALIZATION OF COSMIDS BY NONRADIOACTIVE INSITU HYBRIDIZATION

被引：173
作者：
KIEVITS, T
DAUWERSE, JG
WIEGANT, J
DEVILEE, P
BREUNING, MH
CORNELISSE, CJ
VANOMMEN, GJB
PEARSON, PL
机构：
[1] LEIDEN STATE UNIV,DEPT PATHOL,2333 AL LEIDEN,NETHERLANDS
[2] LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY,2333 AL LEIDEN,NETHERLANDS
来源：
CYTOGENETICS AND CELL GENETICS | 1990年 / 53卷 / 2-3期
关键词：
D O I：
10.1159/000132913
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A rapid method for localizing large numbers of com-pleie cosmids by nonradioactive in situ hybridization is described. The cosmids arc nick translated in the presence of biotin-16- dl IF. mcubuled with an excess of sonicated human DNA. and used as a probe foi in Mlulivbiuh/ Snollu hridiaioiiy delected by successive treatments with FITC-labcled avidin and biolinylated unli-uvidin antihody. cosmids were localized on chromosome 16 in 5 d relative to translocation breakpoints contained in two cell lines Rapid identification of chromosome 16 was achieved by cohybridizalion with a chromosome 16 specific ccntromcric repeal probe. © 1990 S. Karger AG, Basel.
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页码：134 / &
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