EFFECTS OF OXYGEN FREE-RADICALS ON ARTICULAR CHONDROCYTES IN CULTURE - C-MYC AND C-HA-RAS MESSENGER-RNAS AND PROLIFERATION KINETICS

被引：21

作者：

VINCENT, F ^{[1
]}

CORRAL, M ^{[1
]}

DEFER, N ^{[1
]}

ADOLPHE, M ^{[1
]}

机构：

[1] FAC MED COCHIN, CNRS, URA 1147, INST PATHOL MOLEC, F-75674 PARIS 14, FRANCE

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 192卷 / 02期

关键词：

D O I：

10.1016/0014-4827(91)90049-Z

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Since oxygen free radicals are believed to play an important role in cartilage degradation, we studied the effects of these radicals generated by the hypoxanthine-xanthine oxidase system on rabbit articular chondrocytes in culture. Among the damages induced by these radicals, cell proliferation inhibition and G2 arrest were observed. To elucidate the mechanisms involved in this phenomenon, the expression of c-myc and c-Ha-ras genes whose products are associated with cell growth control was studied. Results showed that in chondrocytes, c-myc and c-Ha-ras expression was particularly important during the G1 phase of the cell cycle and that oxygen reactive species, especially H2O2, induced an important decrease of c-myc and c-Ha-ras mRNA levels. Chondrocytes cell cycle analysis revealed an accumulation of cells in G2 phase. It led us to suggest that the chondrocyte cell cycle perturbations observed after oxygen free radicals treatment could be associated with the decrease of c-myc and c-Ha-ras expression. © 1991.

引用

页码：333 / 339

页数：7

共 41 条

[1]

ANDERSON C, 1984, ANAL BIOCHEM, V137, P15

[2]

BARLOGIE B, 1976, CANCER RES, V36, P1176

[3] HYALURONIC-ACID SYNTHESIS IN ARTICULAR-CARTILAGE - AN INHIBITION BY HYDROGEN-PEROXIDE [J].

BATES, EJ ;

LOWTHER, DA ;

JOHNSON, CC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 132 (02) :714-720

[4]

BORS W, 1980, BIOL CLIN ASPECTS SU, P1

[5] CELL-CYCLE CONTROL OF C-MYC BUT NOT C-RAS EXPRESSION IS LOST FOLLOWING CHEMICAL TRANSFORMATION [J].

CAMPISI, J ;

GRAY, HE ;

PARDEE, AB ;

DEAN, M ;

SONENSHEIN, GE .

CELL, 1984, 36 (02) :241-247

[6]

CHAIG EH, 1982, NATURE, V297, P479

[7] EXPRESSION OF C-FOS ONCOGENE DURING HEPATOCARCINOGENESIS, LIVER-REGENERATION AND IN SYNCHRONIZED HTC CELLS [J].

CORRAL, M ;

TICHONICKY, L ;

GUGUENGUILLOUZO, C ;

CORCOS, D ;

RAYMONDJEAN, M ;

PARIS, B ;

KRUH, J ;

DEFER, N .

EXPERIMENTAL CELL RESEARCH, 1985, 160 (02) :427-434

[8] RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN-CELL POPULATIONS [J].

CRISSMAN, HA ;

STEINKAMP, JA .

JOURNAL OF CELL BIOLOGY, 1973, 59 (03) :766-771

[9]

DEAN M, 1986, J BIOL CHEM, V261, P9161

[10] GENE-EXPRESSION DURING THE MAMMALIAN-CELL CYCLE [J].

DENHARDT, DT ;

EDWARDS, DR ;

PARFETT, CLJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 865 (02) :83-125

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