ANALYSIS OF THE TYROSINE PROTEIN-KINASE P56(LCK) EXPRESSED AS A GLUTATHIONE-S-TRANSFERASE FUSION PROTEIN IN SPODOPTERA-FRUGIPERDA CELLS

被引：18
|
作者：
SPANA, C [1 ]
OROURKE, EC [1 ]
BOLEN, JB [1 ]
FARGNOLI, J [1 ]
机构：
[1] BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MOLEC BIOL,PRI,POB 4000,PRINCETON,NJ 08543
来源：
PROTEIN EXPRESSION AND PURIFICATION | 1993年 / 4卷 / 05期
关键词：
D O I：
10.1006/prep.1993.1051
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A baculovirus vector system that expresses cloned DNA sequences as glutathione S-transferase fusion proteins was developed. This system was used to express and purify the lymphocyte-specific tyrosine kinase p56lck. This recombinant p56lck was purified to homogeneity in a single chromatography step using glutathione resin. By SDS gel analysis recombinant p56lck was found to migrate as two species with molecular masses of approximately 56,000 Da. p56lck purified in this manner retained a high level of activity, phosphorylated an exogenous substrate on tyrosine residues, and underwent autophosphorylation on tyrosine residues. The Km (∼0.33 mmol) and Vmax (∼83 pmol min-1 mg-1) values were also determined by using enolase as a substrate. © 1993 Academic Press. All rights reserved.
引用
收藏
页码：390 / 397
页数：8
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