CELL SIZING - A LIGHT SCATTERING PHOTOMETER FOR RAPID VOLUME DETERMINATION

被引：174

作者：

MULLANEY, PF

VANDILLA, MA

COULTER, JR

DEAN, PN

机构：

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 1969年 / 40卷 / 08期

关键词：

D O I：

10.1063/1.1684143

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Theory predicts that small angle light scattering by spherical particles of 5 to 20 μ diam is nearly proportional to volume and insensitive to particle refractive index. A flow system photometer with helium-neon laser light source measures the scattering between 0.5 and 2.0° from individual particles at 104 to 105/min. Volume distributions of mammalian cells and plastic microspheres agree with other independent determinations. © 1969 The American Institute of Physics.

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页码：1029 / &

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