MONOCLONAL ANTIIDIOTYPIC ANTIBODY TO A POTENT THROMBOXANE-A2 RECEPTOR ANTAGONIST AND ITS INTERACTION WITH THROMBOXANE-A2 RECEPTOR

被引：0

作者：

KAN, WM ^{[1
]}

TAI, HH ^{[1
]}

机构：

[1] UNIV KENTUCKY,COLL PHARM,DIV MED CHEM & PHARMACEUT,LEXINGTON,KY 40536

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1993年 / 268卷 / 09期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal anti-idiotypic antibody that interacts with thromboxane A2 receptor was generated using an anti-idiotypic approach. Idiotypic antibodies against a potent receptor antagonist, HS-145, were generated in rabbit. The idiotypic antibodies were then selected by an affinity procedure using SQ29,548-Affi-Gel-102 matrix. The selected idiotypic antibodies were used as surrogate receptor for anti-idiotypic antibody generation. A mouse monoclonal antibody, 3D-9E-12, was generated. It was shown to displace I-125-HS-145 from affinity-purified idiotypic antibodies. It also inhibits I-125-IS-145 binding to thromboxane A2 receptor in human platelet membranes in a dose-dependent manner. Furthermore, it attenuated U46,619-induced increase in [S-35]guanosine 5'-O-(thiotriphosphate) binding and GTPase activity in human platelet membranes. Finally, it inhibited U46,619- but not PAF-induced platelet aggregation. These results indicate that 3D-9E-12 acts as a specific antagonist in the thromboxane A2 receptor.

引用

页码：6364 / 6370

页数：7

共 50 条

[11] ENANTIOSELECTIVE SYNTHESIS OF S-1452, AN ORALLY ACTIVE POTENT THROMBOXANE-A2 RECEPTOR ANTAGONIST [J].

OHTANI, M ;

MATSUURA, T ;

WATANABE, F ;

NARISADA, M .

JOURNAL OF ORGANIC CHEMISTRY, 1991, 56 (06) :2122-2127

[12] SYNTHETIC THROMBOXANE-A2 AND CHARACTERIZATION OF THE THROMBOXANE RECEPTOR USING A NOVEL CLASS OF RECEPTOR ANTAGONISTS [J].

MORTON, HE ;

EVANS, J ;

LEVEILLE, C ;

LORD, A ;

GILLARD, JW .

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 195 :122-MEDI

[13] MITOGENIC EFFECTS OF SEROTONIN AND THROMBOXANE-A2 ON ENDOTHELIAL-CELLS ARE REVERSED BY SEROTONIN AND THROMBOXANE-A2 RECEPTOR ANTAGONISTS [J].

PAKALA, R ;

WILLERSON, JT ;

BENEDICT, CR .

CIRCULATION, 1993, 88 (04) :93-93

[14] ICI-192, 605 - A POTENT, SELECTIVE THROMBOXANE-A2 RECEPTOR ANTAGONIST ON SMOOTH-MUSCLE [J].

JESSUP, CL ;

JESSUP, R ;

WAYNE, M .

BRITISH JOURNAL OF PHARMACOLOGY, 1988, 95 :P675-P675

[15] CLONING AND EXPRESSION OF CDNA FOR A HUMAN THROMBOXANE-A2 RECEPTOR [J].

HIRATA, M ;

HAYASHI, Y ;

USHIKUBI, F ;

YOKOTA, Y ;

KAGEYAMA, R ;

NAKANISHI, S ;

NARUMIYA, S .

NATURE, 1991, 349 (6310) :617-620

[16] THROMBOXANE-A2 PROSTAGLANDIN-H2 RECEPTOR ANTAGONISTS [J].

LEBRETON, GC ;

LIM, CT ;

VAIDYA, CM ;

VENTON, DL .

METHODS IN ENZYMOLOGY, 1990, 187 :406-414

[17] HA-29 - AN INHIBITOR OF THROMBOXANE-A2 FORMATION WITH ANTAGONISM OF THROMBOXANE-A2 PROSTAGLANDIN ENDOPEROXIDE RECEPTOR IN RABBIT PLATELETS [J].

LIN, CH ;

KUO, SC ;

HUANG, LJ ;

HUANG, TF ;

TENG, CM .

THROMBOSIS RESEARCH, 1992, 66 (01) :61-73

[18] EFFECTS OF PRETREATMENT WITH G-619, A DUAL INHIBITOR OF THROMBOXANE-A2 SYNTHASE AND THROMBOXANE-A2 RECEPTOR ANTAGONIST, ON CEREBRAL-ISCHEMIA IN THE MONGOLIAN GERBIL [J].

CALAPAI, G ;

SQUADRITO, F ;

CAMPO, GM ;

MARCIANO, MC ;

MAZZAGLIA, G ;

CILIA, M ;

ALFREDO, M ;

RIZZO, A ;

CRISAFULLI, C ;

SPIGNOLI, G ;

CAPUTI, AP .

NEUROSCIENCE RESEARCH COMMUNICATIONS, 1993, 12 (02) :109-118

[19] SYNTHESIS OF STABLE THROMBOXANE-A2 ANALOGS - PINANE THROMBOXANE-A2 (PTA2) AND CARBOCYCLIC THROMBOXANE-A2 (CTA2) [J].

NICOLAOU, KC ;

MAGOLDA, RL .

METHODS IN ENZYMOLOGY, 1982, 86 :400-409

[20] THE STORY OF THROMBOXANE-A2 [J].

CROSS, PE ;

DICKINSON, RP .

CHEMISTRY IN BRITAIN, 1991, 27 (10) :911-914

← 1 2 3 4 5 →