CARBOHYDRATE SPECIFICITY OF THE RECEPTOR-SITES OF MISTLETOE TOXIC LECTIN-I

被引：37

作者：

WU, AM ^{[1
]}

CHIN, LK ^{[1
]}

FRANZ, H ^{[1
]}

PFULLER, U ^{[1
]}

HERP, A ^{[1
]}

机构：

[1] UNIV WITTEN HERDECKE,INST PHYTOCHEM,BERLIN,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1117卷 / 02期

关键词：

MISTLETOE TOXIC LECTIN-I; HEMAGGLUTINATION-INHIBITION ASSAY; CARBOHYDRATE SPECIFICITY; CARBOHYDRATE COMBINING SITE; CARBOHYDRATE BINDING PROPERTY;

D O I：

10.1016/0304-4165(92)90084-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The carbohydrate specificity of mistletoe toxic lectin-I (ML-I) was studied by haemagglutination-inhibition assay. The results indicated that ML-I has a broad range of affinity for Gal alpha,beta linked sequences. The galabiose (E, Galalpha1 --> 4Gal) sequence, a receptor of the uropathogenic E. coli ligand, was one of the best disaccharide inhibitors tested. The lectin also exhibits affinity for Lac(Galbeta1 --> 4Glc), T(Galbeta1 --> 3GalNAc), I/II(Galbeta1 --> 3/4GlcNAc) and B(Galalpha1 --> 3Gal) sequences. Galalpha1 --> 4Gal and Galbeta1 --> 4Glc are frequently occurring sequences of many glycosphingolipids located at the mammalian cell membranes, such as intestinal and red blood cell membranes, for ligand binding and toxin attachment. This finding provides important information concerning the possible mechanism of intoxication of cells by the mistletoe preparation.

引用

页码：232 / 234

页数：3

共 17 条

[1]

BOCK K, 1988, ADV EXP MED BIOL, V228, P158

[2] ISOLATION AND PROPERTIES OF 3 LECTINS FROM MISTLETOE (VISCUM-ALBUM L) [J].