ISOLATION AND CHARACTERIZATION OF A MONOCLONAL ANTI CK-2 ALPHA-SUBUNIT ANTIBODY OF THE IGG1 SUBCLASS

被引：14
作者：
SCHMIDTSPANIOL, I [1 ]
BOLDYREFF, B [1 ]
ISSINGER, OG [1 ]
机构：
[1] UNIV SAARLAND,INST HUMANGENET,BAU 68,W-6650 HOMBURG,GERMANY
来源：
HYBRIDOMA | 1992年 / 11卷 / 01期
关键词：
D O I：
10.1089/hyb.1992.11.53
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A monoclonal antibody was produced against the recombinant human alpha-subunit of CK-2. The antibody was of the IgG1 subclass and it was isolated from serum-free cell culture media and purified by affinity chromatography on Protein G Sepharose(R). The antibody can be used to detect specifically the CK-2 alpha-subunit in immunoblots from tissue extracts. An ELISA detection test was also established which also allows the identification of the CK-2 alpha-subunit.
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页码：53 / 59
页数：7
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