DETECTION OF CHLAMYDIA-PSITTACI USING DNA PROBES AND THE POLYMERASE CHAIN-REACTION

被引：11
|
作者：
RASMUSSEN, S [1 ]
TIMMS, P [1 ]
机构：
[1] QUEENSLAND UNIV TECHNOL,DEPT MED LAB SCI,CTR MOLEC BIOTECHNOL,GPO BOX 2434,BRISBANE 4001,AUSTRALIA
来源：
FEMS MICROBIOLOGY LETTERS | 1991年 / 77卷 / 2-3期
关键词：
CHLAMYDIA-PSITTACI; DNA HYBRIDIZATION; POLYMERASE CHAIN REACTION;
D O I：
10.1111/j.1574-6968.1991.tb04342.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Fewer than 10(5) elementary bodies of Chlamydia psittaci could be detected by using DNA hybridisation with a plasmid probe specific for avian chlamydial strains. PCR amplification of chlamydial DNA using primers specific for conserved regions of the major outer membrane protein gene enabled the detection of fewer than 10 elementary bodies. DNA could be amplified from 22 of the 24 chlamydial strains tested including avian, feline, ovine, caprine, koala and lymphogranuloma venereum strains.
引用
收藏
页码：169 / 173
页数：5
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