PURIFICATION OF A MAIZE DEHYDRIN

被引：58

作者：

CECCARDI, TL ^{[1
]}

MEYER, NC ^{[1
]}

CLOSE, TJ ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BOT & PLANT SCI,RIVERSIDE,CA 92521

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1994年 / 5卷 / 03期

关键词：

D O I：

10.1006/prep.1994.1040

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A maize dehydrin with an apparent molecular weight of 20 kDa was purified from whole kernels of maize inbred line G50. Kernels were ground in a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89-degrees-C and centrifuged to remove heat-insoluble proteins. The remaining soluble proteins were fractionated in a three-step chromatographic process. Following cation exchange, hydrophobic interaction, and gel filtration chromatography, pure dehydrin samples were obtained. (C) 1994 Academic Press, Inc.

引用

页码：266 / 269

页数：4

共 9 条

[1] A VIEW OF PLANT DEHYDRINS USING ANTIBODIES SPECIFIC TO THE CARBOXY-TERMINAL PEPTIDE [J].

CLOSE, TJ ;

FENTON, RD ;

MOONAN, F .

PLANT MOLECULAR BIOLOGY, 1993, 23 (02) :279-286

[2] AN OSMOTIC-STRESS PROTEIN OF CYANOBACTERIA IS IMMUNOLOGICALLY RELATED TO PLANT DEHYDRINS [J].

CLOSE, TJ ;

LAMMERS, PJ .

PLANT PHYSIOLOGY, 1993, 101 (03) :773-779

[3] A CDNA-BASED COMPARISON OF DEHYDRATION-INDUCED PROTEINS (DEHYDRINS) IN BARLEY AND CORN [J].

CLOSE, TJ ;

KORTT, AA ;

CHANDLER, PM .

PLANT MOLECULAR BIOLOGY, 1989, 13 (01) :95-108

[4] COMMON AMINO-ACID SEQUENCE DOMAINS AMONG THE LEA PROTEINS OF HIGHER-PLANTS [J].

DURE, L ;

CROUCH, M ;

HARADA, J ;

HO, THD ;

MUNDY, J ;

QUATRANO, R ;

THOMAS, T ;

SUNG, ZR .

PLANT MOLECULAR BIOLOGY, 1989, 12 (05) :475-486

[5]

DURE L, 1993, RESPONSE PLANTS CELL, P91

[6] A TOMATO CDNA INDUCIBLE BY SALT STRESS AND ABSCISIC-ACID - NUCLEOTIDE-SEQUENCE AND EXPRESSION PATTERN [J].

GODOY, JA ;

PARDO, JM ;

PINTORTORO, JA .

PLANT MOLECULAR BIOLOGY, 1990, 15 (05) :695-705

[7]

Harlow E, 1988, ANTIBODIES LAB MANUA, P485

[8]

PLANA M, 1991, J BIOL CHEM, V266, P22510

[9] GENE SEQUENCE, DEVELOPMENTAL EXPRESSION, AND PROTEIN-PHOSPHORYLATION OF RAB-17 IN MAIZE [J].

VILARDELL, J ;

GODAY, A ;

FREIRE, MA ;

TORRENT, M ;

MARTINEZ, MC ;

TORNE, JM ;

PAGES, M .

PLANT MOLECULAR BIOLOGY, 1990, 14 (03) :423-432

← 1 →