BACULOVIRUSES - HIGH-LEVEL EXPRESSION IN INSECT CELLS

被引:50
|
作者
MILLER, LK [1 ]
机构
[1] UNIV GEORGIA, ATHENS, GA 30602 USA
关键词
D O I
10.1016/S0959-437X(05)80348-X
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Baculoviruses continue to serve as workhorse vectors for the high-level expression of eukaryotic genes in insect cells; however, numerous researchers are also finding novel uses for these vectors by taking advantage of the unique nature of the viruses and their host cells. Concurrently, the technology involved in constructing and utilizing these vectors is being improved so that the time and effort required to construct expression vectors are reduced.
引用
收藏
页码:97 / 101
页数:5
相关论文
共 50 条
  • [1] HIGH-LEVEL EXPRESSION AND PHOSPHORYLATION OF HEPATITIS-B VIRUS POLYMERASE IN INSECT CELLS WITH RECOMBINANT BACULOVIRUSES
    AYOLA, B
    KANDA, P
    LANFORD, RE
    VIROLOGY, 1993, 194 (01) : 370 - 373
  • [2] Novel vectors for simultaneous high-level dual protein expression in vertebrate and insect cells by recombinant baculoviruses
    Keil, Guenther M.
    Klopfleisch, Constanze
    Giesow, Katrin
    Blohm, Ulrike
    JOURNAL OF VIROLOGICAL METHODS, 2009, 160 (1-2) : 132 - 137
  • [3] HIGH-LEVEL EXPRESSION OF ACTIVE HUMAN CYCLOOXYGENASE-2 IN INSECT CELLS
    CROMLISH, WA
    PAYETTE, P
    CULP, SA
    OUELLET, M
    PERCIVAL, MD
    KENNEDY, BP
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 314 (01) : 193 - 199
  • [4] HIGH-LEVEL EXPRESSION OF FUNCTIONAL GLUTAMATE-RECEPTOR CHANNELS IN INSECT CELLS
    KEINANEN, K
    KOHR, G
    SEEBURG, PH
    LAUKKANEN, ML
    OKERBLOM, C
    BIO-TECHNOLOGY, 1994, 12 (08): : 802 - 806
  • [5] High-level expression of human glycosyltransferases in insect cells as biochemically active form
    Kim, HG
    Yang, SM
    Lee, YC
    Do, SI
    Chung, IS
    Yang, JM
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 305 (03) : 488 - 493
  • [6] Recombinant baculoviruses as expression vectors for insect and mammalian cells
    Kost, TA
    Condreay, JP
    CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (05) : 428 - 433
  • [7] HIGH-LEVEL EXPRESSION OF AMPA-SELECTIVE GLUTAMATE RECEPTOR SUBUNITS IN INSECT CELLS
    KEINANEN, K
    LAUKKANEN, ML
    OKERBLOM, C
    PROTEIN ENGINEERING, 1993, 6 : 80 - 80
  • [8] Rapid, high-level transient expression of papillomavirus-like particles in insect cells
    Benincasa, D
    Silberklang, M
    Mark, GE
    Ludmerer, SW
    BIOTECHNIQUES, 1996, 20 (05) : 890 - +
  • [9] High-level expression of human recombinant carboxypeptidase U in insect- and mammalian cells
    Strömqvist, M
    Andersson, JO
    Edlund, M
    Eiserman, M
    Johansson, T
    Lindberg, M
    Skytt, A
    THROMBOSIS AND HAEMOSTASIS, 1999, : 323 - 323
  • [10] High-level expression of human extracellular superoxide dismutase in Escherichia coli and insect cells
    He, HJ
    Yuan, QS
    Yang, GZ
    Wu, XF
    PROTEIN EXPRESSION AND PURIFICATION, 2002, 24 (01) : 13 - 17