1-METHYL-4-PHENYLPYRIDINIUM (MPP+) INDUCES NADH-DEPENDENT SUPEROXIDE FORMATION AND ENHANCES NADH-DEPENDENT LIPID-PEROXIDATION IN BOVINE HEART SUBMITOCHONDRIAL PARTICLES

被引：319

作者：

HASEGAWA, E ^{[1
]}

TAKESHIGE, K ^{[1
]}

OISHI, T ^{[1
]}

MURAI, Y ^{[1
]}

MINAKAMI, S ^{[1
]}

机构：

[1] KYUSHU UNIV,SCH MED,DEPT BIOCHEM,FUKUOKA 812,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90498-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the effects of 1-methyl-4-phenylpyridinium (MPP+), a metabolite of a parkinsonism-inducing drug, on the superoxide formation and the lipid peroxidation in bovine heart submitochondrial particles. The NADH-supported formation of superoxide radicals was induced by MPP+ at the concentration which is considered to exist in mitochondria of dopamine neurons. The formation increased as the NADH-ubiquinone reductase activity was inhibited by MPP+. The NADH-supported lipid peroxidation by the particles in the presence of ADP-Fe3+ chelate was also enhanced by MPP+ at similar concentrations. The formation was inhibited by succinate and the reduction of endogenous ubiquinone seems to be related to the inhibition. A possibility was discussed that the formation of superoxide anions and the lipid peroxidation may contribute in the cytotoxicity of the drug. © 1990.

引用

页码：1049 / 1055

页数：7

共 33 条

[1] INDUCTION BY 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE OF LIPID-PEROXIDATION INVIVO IN VITAMIN-E DEFICIENT MICE [J].