A METHOD FOR SIMULTANEOUS DETECTION OF FLUORESCENT G-BANDS AND IN-SITU HYBRIDIZATION SIGNALS

被引：71

作者：

HIRAI, M

SUTO, Y

KANOH, M

机构：

[1] Department of Anthropology, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113

来源：

CYTOGENETICS AND CELL GENETICS | 1994年 / 66卷 / 03期

关键词：

D O I：

10.1159/000133687

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Improved techniques for detecting in situ hybridization signals on fluorescent G-bands are described. Prior to hybridization of biotinylated DNA probes, chromosomes with replication G-bands were treated with formaldehyde and denatured with an alkaline solution. These steps preserve chromosome morphology well, and enable us to simultaneously demonstrate fluorescein hybridization signals and G-banded chromosomes stained with propidium iodide. This method is useful for precise chromosomal localization of DNA markers.

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页码：149 / 151

页数：3

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