STEREOCHEMICAL COURSE AND STRUCTURE OF THE PRODUCTS OF THE ENZYMATIC ACTION OF ENDO-1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE FROM BACILLUS-LICHENIFORMIS

被引：66
作者：
MALET, C
JIMENEZBARBERO, J
BERNABE, M
BROSA, C
PLANAS, A
机构：
[1] UNIV RAMON LLULL,INST QUIM SARRIA,CETS,DEPT QUIM ORGAN,E-08017 BARCELONA,SPAIN
[2] CSIC,INST QUIM ORGAN GEN,LARBOHIDRATOS GRP,E-28006 MADRID,SPAIN
来源：
BIOCHEMICAL JOURNAL | 1993年 / 296卷
关键词：
D O I：
10.1042/bj2960753
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The stereochemical course of the reaction catalysed by endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase (EC 3.2.1.73) has been determined by H-1 n.m.r. The enzyme-catalysed hydrolysis of barley beta-glucan proceeds with overall retention of the anomeric configuration, indicating that the enzyme operates through a double-displacement mechanism. The structures of the final oligosaccharide products, 3-beta-O-cellobiosyl D-glucopyranoside and 3-beta-O-cellotriosyl D-glucopyranoside, have been completely assigned by H-1- and C-13-n.m.r. spectroscopy.
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页码：753 / 758
页数：6
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