GENUS-SPECIFIC AND SPECIES-SPECIFIC OLIGONUCLEOTIDE PROBES DERIVED FROM 16S RIBOSOMAL-RNA FOR THE IDENTIFICATION OF VAGOCOCCI

被引:4
|
作者
WILLIAMS, AM [1 ]
COLLINS, MD [1 ]
机构
[1] INST FOOD RES, DEPT MICROBIOL, READING LAB, READING RG2 9AT, ENGLAND
关键词
D O I
10.1111/j.1472-765X.1992.tb00637.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Synthetic oligonucleotide probes specific for vagococci were designed from 16S rRNA sequence data. Molecular hybridizations with PCR-amplified rDNA targets provided an unequivocal means of differentiating vagococci from related lactic acid bacteria (eg. Carnobacterium, Enterococcus, Lactococcus) and identification at the generic and species levels.
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页码:17 / 21
页数:5
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