Extracellular polysaccharides from suspension-cultured cells of Rubus fruticosus. Structure of a galactoglucomannan

被引:1
作者
Chambat, G. [1 ]
Cartier, N. [1 ]
Joseleau, J. P. [1 ]
机构
[1] Ctr Rech Macromol Vegetales, F-38402 St Martin Dheres, France
关键词
D O I
10.1016/S0268-005X(87)80068-1
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Carbohydrate analysis of the cell walls from suspension-cultured cells of Rubus fruticosus between day 10 and day 40 after inoculation revealed an overall increase of the net content of neutral sugars. However the composition of the extracellular polysaccharides showed only discrete variation. A galactoglucomannan containing galactose, glucose and mannose in the ratio 1.0:1.4:1.2 respectively, could be purified by barium hydroxide precipitation both in the extracellular medium and from the alkaline extract of the cell walls. Methylation analysis and (13)C-NMR spectroscopy demonstrated that the extracellular form is the counterpart of the wall polysaccharide.
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页码:555 / 556
页数:2
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