3-DIMENSIONAL OPTICAL-TRANSFER-FUNCTION ANALYSIS FOR A LASER-SCAN FLUORESCENCE MICROSCOPE WITH AN EXTENDED DETECTOR

被引：38

作者：

KAWATA, S

ARIMOTO, R

NAKAMURA, O

机构：

[1] Department of Applied Physics, Osaka University Suita, Osaka

[2] Instruments Division, Nikon Corporation, Yokohama

[3] National Research Laboratory of Metrology, Tsukuba

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 1991年 / 8卷 / 01期

关键词：

D O I：

10.1364/JOSAA.8.000171

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Three-dimensional imaging characteristics of a laser-scan fluorescence microscope with various sizes of detector are quantitatively analyzed through the studies and calculations of the three-dimensional optical transfer function of the microscope. It is found that a detector of an Airy-disk size formed by the objective lens in the detector plane provides both reasonable energy-collection efficiency and three-dimensionally resolved imaging capability. It is also found that a slit detector provides longitudinal resolution of the sample structure similarly to a confocal microscope, while the lateral resolution is approximately the same as that of a conventional microscope.

引用

页码：171 / 175

页数：5

共 15 条

[1]

Awamura D., 1987, Proceedings of the SPIE - The International Society for Optical Engineering, V765, P53, DOI 10.1117/12.940184

[2] 3-DIMENSIONAL RADIOGRAPHIC IMAGING WITH A RESTRICTED VIEW ANGLE [J].